Repeat this cycle to maximize binding to the C18 media. Note: Slow, consistent pipetting is key for high recovery. 5. Washing (Desalting) Aspirate the Wash Solution (0.1% TFA). Dispense to waste. Repeat 3 times to remove salts, buffers, and detergents. 6. Elution Aspirate the Elution Solution (e.g., 60% ACN/0.1% TFA).
Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials C18zip
Repeat using the same elution volume to ensure all peptides are recovered. Repeat this cycle to maximize binding to the C18 media
Dispense the liquid into a clean, sterile microcentrifuge tube. C18zip
Repeat . This prepares the resin to bind the peptides. 4. Sample Binding
Aspirate and dispense your peptide sample through the ZipTip.