: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100.
: It allows for the quantification of intracellular features, such as the translocation of signals into the nucleus or the internalization of particles. Key Protocol Steps Imaging Flow Cytometry: Methods and Protocols
: Systems like the ImageStream use Charge-Coupled Device (CCD) cameras with Time Delay Integration (TDI) to track cells in flow, preventing motion blur and enabling the detection of dim markers. : If internal markers (e
While IFC shares many preparatory steps with conventional flow cytometry, it requires specific optimizations to ensure high-quality imagery. : If internal markers (e.g.
